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173 ʱ䣺2019-11-23
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1ĿĻ򹹽ϣٽתȾϸڣתȾʽҪиתת֬תȾת¼תȾDNAϸ˺󽫻ϵϸ鵱ȥ˱ˮƽ򿽱λת¼йء
2ͬɸѡԼɸѡܹȷĿĻҸˮƽϸȺ(pool
3ڴʱõϸȺеÿϸԸ죬вͬĻλ㡢ϸʣҪои߲ȶԵϸֱͨҪǧĺѡ¡һɸѡ
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1ϡͷLimiting dilution cloningLDCΪͳɱڲ㷺ڿ¡ɸѡҪעǣΪFDA걨ҪLDCϸܶ<0.5ϸÿ׻һLDCͼ֤֤¡ԡϡͷҪԶվиͨɸѡ
2е¡ɸѡϸڰУγɵ¡ϸţͬʱϸΧкӫǵĶ뿹FcƬԽϣһĹмʱܷӫ⡣ʹͼܹԵ¡ϸŵĴСӫǿȽвⶨӶɸѡʢߵĵ¡ϸšԶĻеܹʵԱ趨IJҪϸȡںܹͨԶķʽȡ¡ϸʵԱĹӫɨ衢ͼͿ¡ȡĻ۸񰺹Ϊ걨ҪҪְɸѡһְɸѡһLDCС
3ʽϸǷѡFACSӫǵĶͷڿCHOϸϷڵϸĿܹʽϸǼ⵽ӶѡɸѡڶϸΪʹڵĿܹάϸĤ棬ʹ΢ҽϸԼڵĿ
¡ɸѡ΢ͨͲֶεĸƺdzҪHTRFʱֱӫ⣩ʹýСƷ2.5-5.0μLиͨ384װ壩⣬ԶվĸܹзԱĹ
ͶƱ׬Ǯapp(szyhtm.com)Ӫ:΢׬Ǯapp,GIBCO̥ţѪ,Hyclone̥ţѪ,T-24/DDPϸ,HCT-8/VCRϸ
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